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Biomeda corporation
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Biomeda corporation
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Funakoshi ltd
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Enzo Biochem
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Enzo Biochem
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Histo-Line Laboratories
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ScyTek Inc
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Linaris GmbH
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Daco Corporation
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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds
doi: 10.1186/s13287-015-0075-4
Figure Lengend Snippet: Immunohistochemical analysis of collagen II content within collagen and hyaluronic acid (HA) scaffolds seeded with hypoxia- and normoxia-cultured bone marrow-derived mesenchymal stem cells (BMSCs). BMSCs were isolated/expanded under normoxia or hypoxia, seeded within collagen or HA scaffolds, and subsequently differentiated under normoxia or hypoxia for 14 days in chondrogenic medium. Thereafter, constructs were sectioned at 5-μm thickness, and collagen II immunostaining was performed by using an aminoethylcarbazole-based peroxidase labeling kit. Photomicrographs represent collagen and HA scaffolds seeded with BMSCs from donor Z33.
Article Snippet: Immune-localized antigens were visualized with horse anti-mouse IgG biotinylated secondary antibody (Vectastain; Vector Laboratories Inc., Burlingame, CA, USA) and an
Techniques: Immunohistochemical staining, Cell Culture, Derivative Assay, Isolation, Construct, Immunostaining, Labeling